Isolation and characterization of a chitinase cDNA from the entomopathogenic fungus,Metarhizium anisopliae
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چکیده
منابع مشابه
Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
Entomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open reading frame (ORF) which encodes a protein...
متن کاملIsolation, partial characterization, and cloning of an extracellular chitinase from the entomopathogenic fungus Verticillium lecanii.
The entomopathogenic fungus Verticillium lecanii is a well-known biocontrol agent of fungal phytopathogens, as well as insect pests. A 42-kDa chitinase belonging to family 18 of the glycosyl hydrolases was isolated and partially characterized. Chitinase was purified using successive column chromatography on phenyl-sepharose, DEAE-sepharose, and CM-sepharose. The enzyme showed the highest activi...
متن کاملIsolation, characterization and expression analysis of the ornithine decarboxylase gene (ODC1) of the entomopathogenic fungus, Metarhizium anisopliae.
The gene ODC1, which codes for the ornithine decarboxylase enzyme, was isolated from the entomopathogenic fungus, Metarhizium anisopliae. The deduced amino acid sequence predicted a protein of 447 amino acids with a molecular weight of 49.3 kDa that contained the canonical motifs of ornithine decarboxylases. The ODC1 cDNA sequence was expressed in Escherichia coli cells; radiometric enzyme assa...
متن کاملCharacterization and biocontrol ability of fusion chitinase in Escherichia coli carrying chitinase cDNA from Trichothecium roseum.
The antifungal mechanism of mycoparasitic fungi involves fungal cell wall degrading enzymes such as chitinases. Trichothecium roseum is an important mycoparasitic fungus with significant antifungal ability, but studies on chitinases of T. roseum were poor. Here, we report a novel chitinase cDNA isolated from T. roseum by PCR amplification based on conserved chitinase sequences. Southern blot an...
متن کاملa study on the design of bio-ethanol process from date wastes of sistan and baluchistan province
اتانول کاربردهای متنوعی در صنایع لاستیک سازی، رنگسازی، حلالها ومکمل سوخت خودرو دارد. اتانول برخلاف نفت از جمله مواد تجدیدپذیر محسوب می شود که مشکلات زیست محیطی و آلودگی نیز ایجاد نمی کند. استفاده از اتانول به عنوان مکمل سوختخودروها از جمله مهمترین مصارف صنعتی این ماده بشمار می رود. با توجه به این موضوع تحقیق و توسعه در زمینه تولید اتانول با درجه خلوص بالا در سطح جهان، و نه تنها در کشور های پیشر...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1998
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1998.tb13156.x